Note

for us: These is a list of ideas for when you are done with experiments/acquisition I would like to add.

Closing the setup¶

Organize and save your data¶

Important!

Always back up your data on the server to reduce risk of data lost.

After finishing an experiments/operation with the setup, make sure you transfer your data on the server. The PC dedicated to the experiments does not currently have a backup, so make sure that your data is saved on the Institute cloud.

Reorganize your folders based on the expeiments/annotations, etc (otional but highly recommended).
Save copy of your data on the server. Raw optical map data and experiments are normally stored on the GroupOdening servr under the folder: /Volumes/GroupOdening/OM_data_Bern/raw_data/2024.

Clossing software¶

Checklist of software to close:

Powerlab¶

Simply save and close the current file. Then, just close the application as you normally will do with any other program.

Andor Solis¶

If you have modified the acquisiton setting, the Andor Solis always ask always ask to overwrite the settings to the default one. Please choose not to overwrite.

Napari-omaas¶

Just close the application as you normally will do with any other program.

Picoscope¶

Just close the application as you normally will do with any other program.

Shutdown hardware¶

Make sure you swutdown the following instruments:

Switch off the Camera
Switch off the Powerlab
Switch off the Pacer
Switch off the experiment Computer

Clean the perfusion system¶

The perfusion system is composed of two perfusion circuits:

the aortic block
the recirculation bath

Use destilled and deionized water (18 M ohms) to perfuse exaustively the system. At least 2 L of water for each of the circuits. Finally, make sure to: :

shutdown the water bath
shutdown the pumps
shutdown the oxigen supplier
open all valves and clamsp form the perfusion system.
release the tubing clamps from the 2 pumps

🔨 Work in progress

This tutoriasl is under construction. Soon will be updated.