```{admonition} Note :class: note for us: These is a list of ideas for when you are done with experiments/acquisition I would like to add. - [x] reorganize your folders (optional but recommended) - [x] copy your data to the server - [x] clossing software - [x] clossing hardware - [x] cleanning the perfusion system - [ ] other? ``` # Closing the setup ## Organize and save your data ```{admonition} Important! :class: danger Always back up your data on the server to reduce risk of data lost. ``` After finishing an experiments/operation with the setup, make sure you transfer your data on the server. **The PC dedicated to the experiments does not currently have a backup**, so make sure that your data is saved on the Institute cloud. - [x] Reorganize your folders based on the expeiments/annotations, etc (otional but highly recommended). - [x] Save copy of your data on the server. Raw optical map data and experiments are normally stored on the GroupOdening servr under the folder: `/Volumes/GroupOdening/OM_data_Bern/raw_data/2024`. ## Clossing software Checklist of software to close: ### Powerlab Simply save and close the current file. Then, just close the application as you normally will do with any other program. ### Andor Solis If you have modified the acquisiton setting, the Andor Solis always ask always ask to overwrite the settings to the default one. Please choose not to overwrite. ### Napari-omaas Just close the application as you normally will do with any other program. ### Picoscope Just close the application as you normally will do with any other program. ## Shutdown hardware Make sure you swutdown the following instruments: - [x] Switch off the Camera - [x] Switch off the Powerlab - [x] Switch off the Pacer - [x] Switch off the experiment Computer ## Clean the perfusion system The perfusion system is composed of two perfusion circuits: - the **aortic block** - the **recirculation bath** Use destilled and deionized water (18 M ohms) to perfuse exaustively the system. At least 2 L of water for each of the circuits. Finally, make sure to: : - [x] shutdown the water bath - [x] shutdown the pumps - [x] shutdown the oxigen supplier - [x] open all valves and clamsp form the perfusion system. - [x] release the tubing clamps from the 2 pumps {{under_construction_warn}}